Target
Human ITGAM / CD11b
Synonyms
ITGAM | Antigen CD11b (p170) | CD11B | CD11b antigen | CR3A | Integrin alpha-M | MAC-1 | MO1A | Neutrophil adherence receptor | MAC1A | SLEB6 | CR-3 alpha chain
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Allophycocyanin.
Also available
Unconjugated or conjugated with
Biotin,
FITC,
RPE.
Purification
Protein A purified
Immunogen
Human monocytes
Specificity
Integrin alpha M subunit (CD11b) of about 165 kd.
Applications
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
Five x 105 ficoll prepared human PBMC were washed and pre incubated ~10 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD11b/APC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 10.7% sub population of the cells stained positive with a mean shift of 1.7 log10 fluorescent units when compared to a Mouse IgG1/APC negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD11b antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee