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Antibodies > IGF1R / IGF1 Receptor > LS-C800984
 
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Catalog Number Size Price
LS-C800984-100 100 µl (1 mg/ml) $379 
LS-C800984-200 200 µl (1 mg/ml) $421 
IGF1R / IGF1 Receptor Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IGF1R / IGF1 Receptor Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IGF1R / IGF1 Receptor Antibody - Western blot analysis of IGF1R phosphorylation expression in Insulin treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
IGF1R / IGF1 Receptor Antibody - Western blot analysis of IGF1R phosphorylation expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
IGF1R / IGF1 Receptor Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IGF1R / IGF1 Receptor Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IGF1R / IGF1 Receptor Antibody - Western blot analysis of IGF1R phosphorylation expression in Insulin treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
IGF1R / IGF1 Receptor Antibody - Western blot analysis of IGF1R phosphorylation expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human IGF1R / IGF1 Receptor Antibody (phospho‑Tyr1165,phospho‑Tyr1166, IHC, IF, WB) LS‑C800984

Polyclonal Rabbit anti‑Human IGF1R / IGF1 Receptor Antibody (phospho‑Tyr1165,phospho‑Tyr1166, IHC, IF, WB) LS‑C800984

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Antibody:
IGF1R / IGF1 Receptor Rabbit anti-Human Polyclonal (pTyr1165,pTyr1166) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800984-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IGF1R / IGF1 Receptor Rabbit anti-Human Polyclonal (pTyr1165,pTyr1166) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IGF1 Receptor antibody LS-C800984 is an unconjugated rabbit polyclonal antibody to IGF1 Receptor (IGF1R) (pTyr1165,pTyr1166) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human IGF1R / IGF1 Receptor
Synonyms
IGF1R | CD221 antigen | CD221 | IGF1 Receptor | IGFR | IGF-I receptor | Soluble IGF1R variant 1 | Soluble IGF1R variant 2 | Type 1 IGF receptor | IGFIR | JTK13
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human IGF1R around the phosphorylation site of Tyrosine 1165/Tyrosine 1166.
Epitope
pTyr1165,pTyr1166
Specificity
Phospho-IGF1R (Tyr1165/Tyr1166) Antibody detects endogenous levels of IGF1R only when phosphorylated at Tyrosine 1165/Tyrosine 1166.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGF1R / IGF1 Receptor
P08069 NM_000875 NP_000866.1

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IGF1R / IGF1 Receptor Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IGF1R (Phospho-Tyr1161) Antibody. The picture on the right is blocked with the phospho peptide.
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Product
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Species: Human
Applications: IHC, Western blot, Neutralization
IGF1R / IGF1 Receptor Antibody - Immunohistochemical analysis of IGF1 Receptor (pY1161) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IGF1R / IGF1 Receptor Antibody (phospho‑Tyr1161) LS‑C358783
Species: Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
IGF1R / IGF1 Receptor Antibody - Immunohistochemistry: IGF1 Receptor Antibody - Analysis of IGF1R in human kidney carcinoma using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IGF1R / IGF1 Receptor Antibody LS‑C180145
Species: Human, Mouse, Rat, Primate
Applications: IHC, IHC - Paraffin, ICC, Western blot
See all IGF1R / IGF1 Receptor Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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