Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
TRITC.
Purification
Purified IgG
Immunogen
Purified monoclonal IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Human IgE, Fc specific.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
- Immunodiffusion
- Applications tested for the base form of this product only
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgE in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions may vary widely: for histochemical and cytochemical use usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee