Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
HRP,
TRITC.
Purification
Purified IgG
Immunogen
Purified homogenous IgE isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgE, Fc specific.
Applications
- Immunofluorescence
- ELISA
- Dot Blot
Usage
The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with mouse IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in mouse serum. As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detecting antibody reagent in non-isotopic assay methodology (e. g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee