Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
HRP,
TRITC.
Immunogen
Highly purified normal IgA isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgA, Fc specific.
Usage
The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rat serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee