Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified IgG
Immunogen
Highly purified IgG and pools of homogenous IgA and IgM isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
Purified IgG fraction of polyclonal Swine antiserum to Human immunoglobulins IgG, IgA, IgM, heavy and light chains. Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.
Usage
Applications should be user optimized.
Reconstitution
Reconstitute by adding 1 ml sterile distilled water, spin down to remove insoluble particles, divide into small aliquots, freeze and store at or below -20°C.
Storage
Store at 4°C for short term storage. Aliquot and store at -20°C for long term storage. Avoid freeze/thaw cycles. Prior to use, thaw aliquot slowly at ambient temperature. Spin down and prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Store working dilutions at 4°C, and preferably use the same day. Remove slight precipitation with centrifugation.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee