Reactivity
Yeast, Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Ovalbumin-conjugated, synthetic peptide (SGRGAcKGGKGL-C) corresponding to aa1-10 of yeast histone H4 acetylated on lysine 5, with a C-Terminus cysteine added for conjugation purposes.
Specificity
Recognizes acetyl-Histone H4 (Lys5). Species cross-reactivity: Yeast and human. Broad species cross-reactivity is expected.
Applications
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:1000-1:5000 dilution of this lot detected acetyl-Histone H4 (Lys5) in acid extracts from sodium butyrate treated HeLa cells. Chromatin IP: An independent laboratory has shown that this antibody preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H4 K5R substitution.
Presentation
0.01% Thimerosal
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee