Target
Rat IgG + IgA + IgM
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Purification
Affinity chromatography
Immunogen
Rat IgG, IgA and IgM whole molecules.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat isotypes and chain combinations.
Applications
- Immunofluorescence (1:1000 - 1:5000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee