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Antibodies > DYKDDDDK Tag > LS-C773047
 
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Catalog Number Size Price
LS-C773047-100 100 µg (1 mg/ml) $477 
DYKDDDDK Tag Antibody - Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.

Polyclonal Rabbit anti‑All species DYKDDDDK Tag Antibody (RPE, WB) LS‑C773047

Polyclonal Rabbit anti‑All species DYKDDDDK Tag Antibody (RPE, WB) LS‑C773047

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Antibody:
DYKDDDDK Tag Rabbit Polyclonal (RPE) Antibody
Application:
WB
Reactivity:
All species
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C773047-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rabbit Polyclonal (RPE) Antibody
Application:
WB
Reactivity:
All species
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
DYKDDDDK Tag antibody LS-C773047 is an RPE-conjugated rabbit polyclonal antibody to all species DYKDDDDK Tag. Validated for WB.
Target
All species DYKDDDDK Tag
Host
Rabbit
Reactivity
All species
Clonality
Polyclonal
Conjugations
R. Phycoerythrin. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
Specificity
Binds to DYKDDDDK (FLAG tag) conjugated proteins.
Applications
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
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DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/26/2024
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