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Catalog Number	Size	Price
LS-C756612-10	10 µg	$318
LS-C756612-100	100 µg	$470

Ccl6 Antibody - IHC analysis of CCL6 using anti-CCL6 antibody. CCL6 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CCL6 antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Ccl6 Antibody - Western blot analysis of CCL6 using anti-CCL6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CCL6 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCL6 at approximately 11KD. The expected band size for CCL6 is at 11KD.

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Polyclonal Rabbit anti‑Mouse Ccl6 Antibody (IHC, WB) LS‑C756612

Name: Anti-Ccl6 Antibody | Rabbit anti-Mouse Polyclonal ELISA,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756612-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

Ccl6 Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C756612-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Ccl6 Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Ccl6 antibody LS-C756612 is an unconjugated rabbit polyclonal antibody to Ccl6 from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.

Target

Mouse Ccl6

Synonyms

Ccl6 | C10 | Chemokine (C-C motif) ligand 6 | MRP-1 | SCYA6

See All Ccl6 Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived mouse CCL6 recombinant protein (Position: G22-A116).

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (1 - 5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.