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| Catalog Number | Size | Price |
|---|---|---|
| LS-B652-50 | 50 µl (75 mg/ml) | $515 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser529, IHC, WB) LS‑B652
IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser529, IHC, WB) LS‑B652
Note: This antibody replaces LS-C18901, LS-C9298
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer529) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer529) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NFKB p65 antibody LS-B652 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer529). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 6 publications.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
pSer529
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS529 phosphorylated p65 from mouse, rat or other species has not been determined.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B652 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B652 was determined to be 1:500.
Presentation
Antiserum, 0.1% sodium azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RELA / NFKB p65
Validation
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ RELA / NFKB p65 Antibody (phospho-Ser529) for IHC, WB/Western, ELISA LS-B652 has an LSBio Rating of
Publications (4.5)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (6)
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002 169:6490-7.
The phosphatidylinositol 3-kinase/Akt pathway negatively regulates Nod2-mediated NF-kappaB pathway. Zhao L, Lee JY, Hwang DH. Biochemical pharmacology. 2008 75:1515-25.
Role of NF-kappaB in flow-induced vascular remodeling. Castier Y, Ramkhelawon B, Riou S, Tedgui A, Lehoux S. Antioxidants & redox signaling. 2009 11:1641-9.
Anti-inflammatory and anti-catabolic effects of TENDOACTIVE on human tenocytes in vitro. Shakibaei M, Buhrmann C, Mobasheri A. Histology and histopathology. 2011 26:1173-85.
Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation. Enyong Su, Linwei Zhao, Xiaohang Yang, Binbin Zhu, Yahui Liu, Wen Zhao, Xianpei Wang, Datun Qi, Lijie Zhu, Chuanyu Gao. BMC cardiovascular disorders. 2020 Apr;20:176.
LncRNA H19 aggravates TNF-a-induced inflammatory injury via TAK1 pathway in MH7A cells. Jialiang Yang, Yixuan Li, Lili Wang, Zhenchun Zhang, Zunzhong Li, Qian Jia. BioFactors (Oxford, England). 2020 September;46:813-820.
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Species:
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Applications:
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Applications:
IHC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
